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enzyme) Along with the adjacent subunit ion-pairs together with the phosphate moiety within your substrate (ten). It is not likely this arginine residue could lead on your diminution in k

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with various substrate analogs. The response procedure is of curiosity simply because NagA from bacterial resources is identified with equally a couple of divalent metallic ions from your Energetic Internet-internet site.

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Mutation of Y223 into a phenylalanine residue resulted inside of a mutant with almost the exact same catalytic action because the wild type enzyme and so this residue just isn't really substantial for that binding of substrate in NagA.

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